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lc3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc lc3
    Lc3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lc3+a+b/LC3A%2FB+Antibody/pm41934864-114-58-60
    Average 97 stars, based on 4895 article reviews
    lc3 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    other:

    Article Title: Halofuginone is a Molecular Glue Degrader of Integrin β4.
    Article Snippet: Anti-rabbit IgG (7074), Anti-mouse IgG (7076), Integrin β4 (14803), LC3 A/B (12741), cleaved PARP (5625), HA-tag (3724), caspase 3 (9662), cleaved caspase 3 (9661), Bcl-2 (15071), YAP (14074), p-YAP (13008), MST 1 (3682), LATS 1 (3477), p-MST1 (49332), CAND1 (8759), Cul1 (4995), Cul3 (10450), Rbx1 (11922) were purchased from Cell Signaling Technology.

    Article Title: NoxO1 promotes endosome formation and reduces intracellular vesicle processing
    Article Snippet: LC3 A/B , rabbit , Cell Signaling , #4108.

    Article Title: Targeted Lipid Metabolism Screening Uncovers Regulatory Effects on the STING Immune Response in Mevalonate, Eicosanoid and Fatty Acid Pathways
    Article Snippet: Antibodies directed against IRF3 (clone D83B9), P-IRF3 (clone 4D4G), STING (clone D1V5L), P-STING ser 366 (clone D8F4W), P-TBK-1 ser 172 (clone D52C2), p65 (clone D14E12), P-p65 ser 536 (clone 93H1), LC3 A/B (clone D3U4C) and GAPDH (clone 14C10) were purchased from Cell Signaling (Danvers, MA).

    Western Blot:

    Article Title: Hesperetin alleviates neuronal pyroptosis by promoting mitophagy via DHCR24/BACE1 signaling pathway after subarachnoid hemorrhage in mice.
    Article Snippet: Caspase-1 Cysteinyl aspartate-specific proteinase-1 DHCR24 3-β-Hydroxysteroid-Δ-24-reductase DCFH-DA 2′,7′-Dichlorofluorescin diacetate DAPI 4’,6-Diamidino-2-phenylindole EBI Early brain injury ECL Electro-chemi-luminescence EPM The Elevated Plus Maze GSDMD Gasdermin D IL-18 Interleukin-18 NOR The New Object Recognition test OFT Open field test OxyHb Oxyhemoglobin PBS Phosphate buffer saline PVDF Polyvinylidene Fluoride PFA Paraformaldehyde ROS Reactive oxygen species SAH Subarachnoid hemorrhage TME Transmission electron microscope WB Western blot Abbreviations ASC Apoptosis associated with speck-like proteins BACE1 Beta-site amyloid precursor protein cleaving enzyme BBB Blood–brain barrier

    Article Title: Selective Elimination of Myeloid HIV Reservoirs by Targeting Pro-survival Pathways.
    Article Snippet: .. Macrophages with or without HIV-1 infection as above were stimulated for 24 h with 0.1 μM IDB or vehicle control, followed by Western blot analyses as described.7 Cell lysates were used for SDS-PAGE and Western blot analyses by probing with monoclonal antibodies to CASPASE-9 (1:1,000, M054-3, Medical & Biological Laboratories) and CASPASE-7 (1:1,000, M053-3, Medical & Biological Laboratories), cleaved CASPASE-9 (1:1,000, 52873s, Cell Signaling), CASPASE-3 (1:1,000, 9665s, Cell Signaling), cleaved CASPASE-3 (1:1,000, 9501s, Cell Signaling), CASPASE-6 (1:1,000, 9762s, Cell Signaling), BCL-2 (1:1,000, 4223s, Cell Signaling), BCL-xL (1:1,000, 2762s, Cell Signaling), MCL-1 (1:1,000, 5453s, Cell Signaling), BAK (1:1,000, 12105s, Cell Signaling), BAX (1:1,000, 2772s, Cell Signaling), LC3 A/B (1:1000, 4108s, Cell Signaling) or monoclonal anti--Actin (1:5,000, sc-47778, Santa Cruz Biotechnology). .. The blots were then incubated with HRP-conjugated goat anti-Mouse IgG1 (1:5,000, 1070-05, Southern Biotech) or HRP-conjugated goat anti-Rabbit IgG (1:5,000, ab6721, Abcam) and developed using SuperSignal West Dura Extended Duration Substrate (ThermoFisher).

    Bioprocessing:

    Article Title: Autophagy-Related Proteins Influence Mouse Epididymal Sperm Motility
    Article Snippet: .. The primary antibodies included the following: mouse monoclonal antibodies against beta Actin Antibody ((C4) sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA); rabbit polyclonal antibodies against mTOR (#2972s; Cell Signaling Technology, Danvers, MA, USA) and LC3 A/B (#4108; Cell Signaling Technology, MA, USA); and rabbit monoclonal antibodies against SQSTM1/p62 ((D6M5X) #23214; Cell Signaling Technology, MA, USA). .. HRP secondary antibodies were also used, including Goat Anti-Rabbit IgG H&L (HRP) (Ab6721; Abcam, Cambridge, UK) and Rabbit Anti-Mouse IgG H&L (HRP) (Ab6728; Abcam, Cambridge, UK).

    Article Title: Selective Elimination of Myeloid HIV Reservoirs by Targeting Pro-survival Pathways.
    Article Snippet: .. Macrophages with or without HIV-1 infection as above were stimulated for 24 h with 0.1 μM IDB or vehicle control, followed by Western blot analyses as described.7 Cell lysates were used for SDS-PAGE and Western blot analyses by probing with monoclonal antibodies to CASPASE-9 (1:1,000, M054-3, Medical & Biological Laboratories) and CASPASE-7 (1:1,000, M053-3, Medical & Biological Laboratories), cleaved CASPASE-9 (1:1,000, 52873s, Cell Signaling), CASPASE-3 (1:1,000, 9665s, Cell Signaling), cleaved CASPASE-3 (1:1,000, 9501s, Cell Signaling), CASPASE-6 (1:1,000, 9762s, Cell Signaling), BCL-2 (1:1,000, 4223s, Cell Signaling), BCL-xL (1:1,000, 2762s, Cell Signaling), MCL-1 (1:1,000, 5453s, Cell Signaling), BAK (1:1,000, 12105s, Cell Signaling), BAX (1:1,000, 2772s, Cell Signaling), LC3 A/B (1:1000, 4108s, Cell Signaling) or monoclonal anti--Actin (1:5,000, sc-47778, Santa Cruz Biotechnology). .. The blots were then incubated with HRP-conjugated goat anti-Mouse IgG1 (1:5,000, 1070-05, Southern Biotech) or HRP-conjugated goat anti-Rabbit IgG (1:5,000, ab6721, Abcam) and developed using SuperSignal West Dura Extended Duration Substrate (ThermoFisher).

    Incubation:

    Article Title: Inhibition of Autophagy Reveals ATR Protein Kinase as a Key Mediator of Cisplatin Sensitivity in Osteosarcoma
    Article Snippet: .. Nitrocellulose membranes were incubated with primary antibody for 1 h (1:1000; Cell Signaling Technology, 12741, LC3 A/B; 5114, p62; 8558, ATG7; 12994, ATG5, p53 (7F5), 2527, Phospho‐p53 (S15), 9284) at 4°C. .. Binding of HRP‐linked secondary antibody (1:2000; Cell Signaling Technology, 7074) was visualized by enhanced chemiluminescence using Li‐Cor Odyssey Fc machine and quantified using Image Studio (Licor, Cambridge, UK).

    Infection:

    Article Title: Selective Elimination of Myeloid HIV Reservoirs by Targeting Pro-survival Pathways.
    Article Snippet: .. Macrophages with or without HIV-1 infection as above were stimulated for 24 h with 0.1 μM IDB or vehicle control, followed by Western blot analyses as described.7 Cell lysates were used for SDS-PAGE and Western blot analyses by probing with monoclonal antibodies to CASPASE-9 (1:1,000, M054-3, Medical & Biological Laboratories) and CASPASE-7 (1:1,000, M053-3, Medical & Biological Laboratories), cleaved CASPASE-9 (1:1,000, 52873s, Cell Signaling), CASPASE-3 (1:1,000, 9665s, Cell Signaling), cleaved CASPASE-3 (1:1,000, 9501s, Cell Signaling), CASPASE-6 (1:1,000, 9762s, Cell Signaling), BCL-2 (1:1,000, 4223s, Cell Signaling), BCL-xL (1:1,000, 2762s, Cell Signaling), MCL-1 (1:1,000, 5453s, Cell Signaling), BAK (1:1,000, 12105s, Cell Signaling), BAX (1:1,000, 2772s, Cell Signaling), LC3 A/B (1:1000, 4108s, Cell Signaling) or monoclonal anti--Actin (1:5,000, sc-47778, Santa Cruz Biotechnology). .. The blots were then incubated with HRP-conjugated goat anti-Mouse IgG1 (1:5,000, 1070-05, Southern Biotech) or HRP-conjugated goat anti-Rabbit IgG (1:5,000, ab6721, Abcam) and developed using SuperSignal West Dura Extended Duration Substrate (ThermoFisher).

    Control:

    Article Title: Selective Elimination of Myeloid HIV Reservoirs by Targeting Pro-survival Pathways.
    Article Snippet: .. Macrophages with or without HIV-1 infection as above were stimulated for 24 h with 0.1 μM IDB or vehicle control, followed by Western blot analyses as described.7 Cell lysates were used for SDS-PAGE and Western blot analyses by probing with monoclonal antibodies to CASPASE-9 (1:1,000, M054-3, Medical & Biological Laboratories) and CASPASE-7 (1:1,000, M053-3, Medical & Biological Laboratories), cleaved CASPASE-9 (1:1,000, 52873s, Cell Signaling), CASPASE-3 (1:1,000, 9665s, Cell Signaling), cleaved CASPASE-3 (1:1,000, 9501s, Cell Signaling), CASPASE-6 (1:1,000, 9762s, Cell Signaling), BCL-2 (1:1,000, 4223s, Cell Signaling), BCL-xL (1:1,000, 2762s, Cell Signaling), MCL-1 (1:1,000, 5453s, Cell Signaling), BAK (1:1,000, 12105s, Cell Signaling), BAX (1:1,000, 2772s, Cell Signaling), LC3 A/B (1:1000, 4108s, Cell Signaling) or monoclonal anti--Actin (1:5,000, sc-47778, Santa Cruz Biotechnology). .. The blots were then incubated with HRP-conjugated goat anti-Mouse IgG1 (1:5,000, 1070-05, Southern Biotech) or HRP-conjugated goat anti-Rabbit IgG (1:5,000, ab6721, Abcam) and developed using SuperSignal West Dura Extended Duration Substrate (ThermoFisher).

    SDS Page:

    Article Title: Selective Elimination of Myeloid HIV Reservoirs by Targeting Pro-survival Pathways.
    Article Snippet: .. Macrophages with or without HIV-1 infection as above were stimulated for 24 h with 0.1 μM IDB or vehicle control, followed by Western blot analyses as described.7 Cell lysates were used for SDS-PAGE and Western blot analyses by probing with monoclonal antibodies to CASPASE-9 (1:1,000, M054-3, Medical & Biological Laboratories) and CASPASE-7 (1:1,000, M053-3, Medical & Biological Laboratories), cleaved CASPASE-9 (1:1,000, 52873s, Cell Signaling), CASPASE-3 (1:1,000, 9665s, Cell Signaling), cleaved CASPASE-3 (1:1,000, 9501s, Cell Signaling), CASPASE-6 (1:1,000, 9762s, Cell Signaling), BCL-2 (1:1,000, 4223s, Cell Signaling), BCL-xL (1:1,000, 2762s, Cell Signaling), MCL-1 (1:1,000, 5453s, Cell Signaling), BAK (1:1,000, 12105s, Cell Signaling), BAX (1:1,000, 2772s, Cell Signaling), LC3 A/B (1:1000, 4108s, Cell Signaling) or monoclonal anti--Actin (1:5,000, sc-47778, Santa Cruz Biotechnology). .. The blots were then incubated with HRP-conjugated goat anti-Mouse IgG1 (1:5,000, 1070-05, Southern Biotech) or HRP-conjugated goat anti-Rabbit IgG (1:5,000, ab6721, Abcam) and developed using SuperSignal West Dura Extended Duration Substrate (ThermoFisher).



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    ANXA3 did not regulate myocardial free fatty acid uptake or lipolysis but positively modulated macroautophagy in T2DM mice. (A-C) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of db/m and db/db mice analyzed by Western blot, n = 4. (D-F) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of mice analyzed by Western blot, n = 4. (G-I) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6. (J-L) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6-8. The data were presented as the mean ± SD. ∗ P < 0.05 versus the db/m group or the db/db + OE-GFP group; ∗∗ P < 0.01 versus the db/m group or the vehicle group or the OE-NC + vehicle group; ∗∗∗ P < 0.001 versus the vehicle group or the OE-NC + vehicle group; # P < 0.05 versus the OE-NC + HGPA group; ### P < 0.001 versus the OE-NC + HGPA group.

    Journal: Redox Biology

    Article Title: YY1 nitration participates in DbCM cardiomyocyte lipotoxicity by inhibiting ANXA3 -induced microlipophagy

    doi: 10.1016/j.redox.2026.104085

    Figure Lengend Snippet: ANXA3 did not regulate myocardial free fatty acid uptake or lipolysis but positively modulated macroautophagy in T2DM mice. (A-C) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of db/m and db/db mice analyzed by Western blot, n = 4. (D-F) Relative p62 protein expression level and LC3 II/I ratio in heart tissues of mice analyzed by Western blot, n = 4. (G-I) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6. (J-L) Relative p62 protein expression level and LC3 II/I ratio in AC16 cardiomyocytes analyzed by Western blot, n = 6-8. The data were presented as the mean ± SD. ∗ P < 0.05 versus the db/m group or the db/db + OE-GFP group; ∗∗ P < 0.01 versus the db/m group or the vehicle group or the OE-NC + vehicle group; ∗∗∗ P < 0.001 versus the vehicle group or the OE-NC + vehicle group; # P < 0.05 versus the OE-NC + HGPA group; ### P < 0.001 versus the OE-NC + HGPA group.

    Article Snippet: Blocked with 5% (w/v) non-fat-dried milkat room temperature for 1 h. Then the membranes were incubated with the anti-ANXA3 antibody (Proteintech, 11804-1-AP; 1:1000 [v/v]), the anti-YY1 antibody (Proteintech, 22156-1-AP; 1:1000 [v/v]) the anti-PLIN2 antibody (Proteintech, 15294-1-AP; 1:1000 [v/v]), the anti-SQSTM1/p62 antibody (Cell Signaling Technology, 23214; 1:1000 [v/v]), the anti-LC3 antibody (Cell Signaling Technology, 12741; 1:1000 [v/v]), the anti-Rab7 antibody (Proteintech, 55469-1-AP; 1:1000 [v/v])or the rabbit anti-α-Tubulin antibody (ABclonal, AC031; 1:1000 [v/v])overnight at 4 °C.

    Techniques: Expressing, Western Blot