polyclonal antibodies against lc3  (Cell Signaling Technology Inc)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Epimedium Brevicornu and Curculigo orchioides inhibit osteoclast autophagy by degrading the level of miRNA-199 to regulate the mTOR signaling pathway

doi: 10.1186/s13018-025-06043-0

Figure Lengend Snippet: EBCO inhibited the expression of autophagy-related proteins in osteoclasts. The protein expressions of Beclin-1 and LC3 were detected by Western blot. GAPDH was used as the internal control. n = 3, * P < 0.05, ** P < 0.01. MHY1485 (2 µM): mTOR agonist. Dactolisib (10 nM): mTOR inhibitor

Article Snippet: The membranes were then blocked by 5% fat-free milk and incubated respectively with light chain 3 (LC3) A/B antibody (14,16 kDa, 1:1000; 4108, Cell Signaling Technology, USA), Beclin-1 antibody (60 kDa, 1:1000; 3495, Cell Signaling Technology, USA), phosphorylated mTOR (p-mTOR) antibody (289 kDa, 1:1000; 5536, Cell Signaling Technology, USA), mTOR antibody (289 kDa, 1:1000; 2983, Cell Signaling Technology, USA), p70S6 kinase (p70S6K) antibody (70 kDa, 1:1000; 34475, Cell Signaling Technology, USA), phosphorylated p70S6K (p-p70S6K) antibody (70 kDa, 1:1000; 9234, Cell Signaling Technology, USA), β-Actin antibody (42 kDa, 1:5000; AF7018, Affinity Biosciences, China) and anti-GAPDH (36 kDa, 1:5000; Mab5465, Multi Sciences, China) at 4°C overnight.

Techniques: Expressing, Western Blot, Control

Journal: PLOS One

Article Title: The protective effect of iron isomaltoside on myocardial ischemia-reperfusion injury via the suppression of KLF4/NF-κB signaling

doi: 10.1371/journal.pone.0323247

Figure Lengend Snippet: (A) qRT-PCR analyses of pro-inflammatory factorsin rat heart tissues of each groups (n = 5). (B) Representative picture of western blot and density analysis for pro-inflammatory factorsin rat heart tissues of each groups (n = 5). (C) Representative picture of western blot and density analysis for Beclin 1 and LC3 in rat heart tissues of each groups (n = 5). Full-length blots/gels are presented in supplementary Fig 2B and supplementary Fig 2C. (D) Immunohistochemistry results of LC3 in indicated groupss (n = 5). **p < 0.01 vs. Sham; #p < 0.05, ##p < 0.01 vs. I/R.

Article Snippet: Following the washing process, the sections were left to incubate overnight at a temperature of 4°C with the LC3 antibody (1:100; #4108, Cell Signaling, USA).

Techniques: Quantitative RT-PCR, Western Blot, Immunohistochemistry

Journal: PLOS One

Article Title: The protective effect of iron isomaltoside on myocardial ischemia-reperfusion injury via the suppression of KLF4/NF-κB signaling

doi: 10.1371/journal.pone.0323247

Figure Lengend Snippet: (A) Representative picture of western blot and density analysis for Beclin 1 and LC3 in H9C2 cells of each group (n = 5). Full-length blots/gels are presented in supplementary Fig 4A. (B) Immunofluorescence results of H9C2 cells in each group (n = 5). ( C ) qRT-PCR analyses of pro-inflammatory factorsin each group (n = 5). ** p < 0.01 vs. Control; # p < 0.05, ## p < 0.01 vs. H/R.

Article Snippet: Following the washing process, the sections were left to incubate overnight at a temperature of 4°C with the LC3 antibody (1:100; #4108, Cell Signaling, USA).

Techniques: Western Blot, Immunofluorescence, Quantitative RT-PCR, Control

Journal: PLOS One

Article Title: The protective effect of iron isomaltoside on myocardial ischemia-reperfusion injury via the suppression of KLF4/NF-κB signaling

doi: 10.1371/journal.pone.0323247

Figure Lengend Snippet: After transfection of pcDNA3.1-KLF4, the subsequent experiments were performed . (A) Apoptosis in indicated groups (n = 5) was detected applying a flow cytometer. (B) ATP level of H9C2 cells in indicated groups (n = 5). (C) MMP of H9C2 cells in indicated groups (n = 5)was tested with a flow cytometer. (D) Representative picture of western blot and density analysis for Beclin 1 and LC3 in H9C2 cells of each group (n = 5). Full-length blots/gels are presented in supplementary Fig 6D. ( E ) qRT-PCR analyses of pro-inflammatory factors in indicated groups (n = 5). ** p < 0.01 vs. H/R; ## p < 0.01 vs. H/R + IIM 1.

Article Snippet: Following the washing process, the sections were left to incubate overnight at a temperature of 4°C with the LC3 antibody (1:100; #4108, Cell Signaling, USA).

Techniques: Transfection, Flow Cytometry, Western Blot, Quantitative RT-PCR